Osmotic mutants

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Mutants in the S. cerevisiae PKC1 gene display a cell cycle-specific osmotic stability defect

The PKC1 gene of Saccharomyces cerevisiae encodes a homologue of the Ca(2+)-dependent isozymes of mammalian protein kinase C (Levin, D.cE., F. O. Fields, R. Kunisawa, J. M. Bishop, and J. Thorner. 1990. Cell. 62:213-224). Cells depleted of the PKC1 gene product display a uniform phenotype, a behavior indicating a defect in the cell division cycle (cdc). These cells arrest division after DNA rep...

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Isolation, characterization, and genetic analysis of osmotic mutants of Neurospora crassa.

O S M O T I C (os) mutants of N . crassa are those whose growth is inhibited by high osmolalities. The usual operational definition is inhibition of growth of the mutant strains by the addition of 4% NaCl to the growth medium (PERKINS 1959). Prior to the present investigation, a group of os mutants had been mapped on linkage group IR, at approximately 90 map units from the centromere (PERKINS 1...

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Multicopy suppression by asd gene and osmotic stress-dependent complementation by heterologous proA in proA mutants.

Auxotrophic proA mutants of Escherichia coli were complemented by two different classes of Corynebacterium glutamicum genes. One of these was the asd gene. The E. coli asd gene also complements the same proA alleles. Complementation of proA by the asd+ gene requires a high asd dosage and the proB and the proC gene products. The reciprocal complementation pattern (asd by the proA+ gene) was not ...

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The rate of osmotic downshock determines the survival probability of bacterial mechanosensitive channel mutants.

Mechanosensitive (MS) channels allow cells to sense and respond to environmental changes. In bacteria, these channels are believed to protect against an osmotic shock. The physiological function of these channels has been characterized primarily by a standardized assay, where aliquots of batch-cultured cells are rapidly pipetted into a hypotonic medium. Under this method, it has been inferred m...

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Effect of the osmotic pressure of the growth medium on fabB mutants of Escherichia coli.

Temperature-sensitive, unsaturated fatty acid (fabB) auxotrophs of Escherichia coli can grow at the restrictive temperature in the absence of unsaturated fatty acid in a medium with a high osmotic pressure. If a mutant culture was starved for unsaturated fatty acids and harvested just before the lysis started, the fatty acid composition of the cells was the same as that of cells grown until lat...

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ژورنال

عنوان ژورنال: Fungal Genetics Reports

سال: 1974

ISSN: 1941-4765

DOI: 10.4148/1941-4765.1807